100 fmol 1536-well (8 µL) 0. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. For wavelengths in the UV range above 220 nm,. ALP 003-Altitude Lab Emergency Plan. Keywords: spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software,. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 1 x 10-15. 1. spectramax, i series, i3x, spectramax i3x multi-mode detection platform, spectramax i3x, ce, spectramax i3x multi-mode microplate reader, spectramax id, spectramax id5, spectramax id3 multi-mode microplate reader, spectramax id3, sof Created Date: 8/20/2020 9:11:23 AM The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SoftMax® Touch Software is installed in. SpectraMax® iD5. i3. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The corrected absorbance is within 5% of the value. 25 M ). The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. DNA standards ranging from 50 pg/mL to 1 μg/mL were detected using the Quant-iT PicoGreen dsDNA Assay Kit and SpectraMax readers (data from the SpectraMax iD5 reader are shown, but other SpectraMax readers gave similar results). 1. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader,. 2 only). SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. SpectraMax iD5 Multi-Mode Microplate Re. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. With optimized reagents, option to operate the. 7°C (gradient heating design). AquaMax 2000 and 4000 Microplate Washers. Figure 1. SpectraMax Mini: 17. Assay setup for a 384-well low-volume plate. 312 gain calibration failed. Paradigm spectramax manualslibSpectramax id5 Molecular devices spectramax plus 384 user manual pdf downloadShaking spectramax id3 frequently. 1. A 1:10 serial dilution of ATP was prepared in medium. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. 6 mM NaCl in a total reaction volume of 160 μL. Specific lysis was calculated as follows: 100 × (experimental release − spontaneous release)/(maximum release − spontaneous release)The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. 1. , San Jose, CA,. Cet appareil ISM est confomre à la norme NMB-001 du Canada. The SpectraMax iD5 reader can perform fluorescence polarization (FP) allowing measurement of Valita Titer plates with automated interpolation of results for each sample analyzed against the assay-included standard curve. 8. Our SpectraMax iD3 and iD5 readers are ideal for busy, multi-user, multi-application labs. Abstract. 2. book Page 1 Wednesday, July 7, 2010 2:09 PMWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. The analysis capabilities of SoftMax Pro provide quantitation in an easy-to-read, user-The SpectraMax® iD5 Multi-Mode Microplate Reader with ultra-cooled photomultiplier tube (PMT) offers highly sensitive luminescence detection of the Lumit Dx SARS-CoV-2 Immunoassay. QuickSync. In addition, the SpectraMax iD5 can be expanded to. In addition, the iD5 reader measures TRF and FP. The drawing shows a standard microplate of 15 mm height covered with a lid. whitepaper Application note: Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. Other technologies such as imaging. spr * SpectraMax ABS Plus SpectraTest ABS2 v1. Materials. [email protected] x 10-15. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Plate reader. 314 low lamp level warning. 5. plates and fluorescence spectra were measured on Molecular Devices SpectraMax iD5 Microplate Reader with excitation wavelength at 470 nm. 1. , San Jose, CA, USA). SpectraMax M5e: 118. 8. BMG Labtech - FLUOSTAR OMEGA. Lower limits of detection for ATP determined using the ATPlite . 50. A wide linear dynamic range ensures accurate assay results across a broad span of sample types. 8. SpectraMax L: 36. SpectraMax iD5: 81. The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. book Page 1 Wednesday, July 7, 2010 2:09 PM With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. 1 x 10-15. 6% for the on-board optics (monochromators). The SpectraMax® iD5 Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Saturation binding curves measured on the SpectraMax iD5 reader. logistic, and EC. The SpectraMax iD5 reader performed all analytical measurements required for this screening platform. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. In addition, the SpectraMax iD5 reader can be expanded to include bottom-read luminescence, TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. See SpectraMax® Microplate Reader User Manual Download Page. application or budget. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. The measurement conditions should be set up in the SoftMax® Pro software according to the following indications: Setup TRF moduleSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. SpectraMax iD5 多功能微孔板读板机具 有光吸收检测功能，可以进行蛋白定量 检测。下面，我们介绍一下如何用 SpectraMax iD5 配套 SoftMax®Pro 软件， 用 Pierce BCA 蛋白检测试剂盒和 Pierce Rapid Gold BCA 蛋白检测试剂盒进行检 测。以上试剂盒均包括标准品牛血清白蛋 The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 4. ALP 006-Altitude Lab Spill Response. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as a 10-fold serial dilution series . 5. Consistent results are obtained over time offering workflow flexibility. LoadingThe SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Price range: $ 0 - $ 0. 00800 665 32860. 100 fmol 384-well (75 µL) 0. Kysy tuotteesta. NF-κB is a ‘master regulator’ of gene. A brief overview of the SpectraMax iD5 Hybrid Microplate Reader. Prepare your i3/i3x instrument for shipment. style. Tips for keeping your SpectraMax® iD5 in good order: Always turn the power off and disconnect the power cord from the main power source before you use liquids to clean the instrument. 304 signal level saturation. マルチモードマイクロプレートリーダーSpectraMax® iD5 は、吸光、蛍光、発光、時間分解蛍光（TRF）および蛍光偏光（FP）測定モードを内蔵するプレートリーダです。タッチパネルや近距離無線通信など最先端の機能を搭載したマルチモードプレートリーダです。 The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. We would like to show you a description here but the site won’t allow us. B) The enhancing effect of NECA was offset by A2AR antagonist, but not by antagonists specific. Each experiment was repeated three times. 0200-0729. Genscript cPass SARS-CoV-2 Neutralization Antibody Detection. 41 nM were calculated using SoftMax Pro Software. 1. SpectraMax iD5: 81. Spectranet customer portalORDER DOCUMENTATION Unparalleled performance on a personalized platform. 2. Talk to an expert. SpectraMax Mini: 17. 5. We offer over 1000 different promotional items everything from coffee mugs to t-shirts that can be. SpectraMax® iD3 SpectraMax® iD5 SpectraMax® i3x ABSFL LUMI CELL IMAGING WESTERN BLOT INJECTOR TRF FP ALPHA-SCREEN FRET ABS. 1. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013iD5. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 7 mm for all shake intensities. spr SpectraMax iD5 SpectraTest ABS2 v1. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. 1. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. SpectraMaxi3Multi. Sunnyvale, California -- October 5, 2017. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. また、 プレートリーダのメーカー15社一覧 や 企業ランキング も掲載しておりますので是非ご覧ください。. In addition, the SpectraMax iD5吸収度マイクロプレートリーダー SpectraMax® Mini. 303 unable to cal dark current. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. 相比较于传统光栅读板机，特殊滤光片对 红外 (IR) 或红敏型 (Red-Shifed) 实验检测具有10-40倍的. 2 x 10-14. 7°C and the bottom to 36. SpectraMax® iD3 SpectraMax® iD5 SpectraMax® i3x ABSFL LUMI CELL IMAGING WESTERN BLOT INJECTOR TRF FP ALPHA-SCREEN FRET ABS. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. We are a Canadian custom apparel and promotional company here in Victoria, BC. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. 1step assay with SpectraMax microplate readers. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3 allows users to customize workflow to perfectly. 1. In addition, the SpectraMax® iD5 can be expanded to include bottom. Features. Amplitude : 1. The SpectraMax iD3/iD5 readers use SoftMax ® Pro Data Acquisition & Analysis. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. The calculated LOQ values were 0. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. The absorbance was measured using Spectra. 主机内置光栅和滤光片双光路系统，可随意组合进行检测优化，实现高灵活性和高灵敏度的结合。. 概要 - マルチプレートリーダーSpectraMax® iD3/iD5. 3. 1 x 10-15. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. Binding and Inhibition. 4. iD5. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. 1. SoftMax® Pro Software will automatically generate a standard curve and calculate target antigen concentration in unknown samples. 使用SpectraMax iD5酶标仪的四光栅系 统进行波长选择的荧光检测与使用专门用 于Alexa594示踪剂的滤光片对的荧光检 测进行了比较，SpectraMax iD3酶标仪 仅使用四光栅系统检测荧光。 表1 SpectraMax iD3/iD5酶标仪的推荐设置。使用新批次的微孔板和测定体. M1 cells were cultured in black clear-bottom 96-well plates and incubated with 10 μm DHE in the presence or absence of 200 U/mL PEG-SOD (Sigma) in HBSS. A general workflow for sample prep and ELISA is. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The p53 pathway activator nutlin-3 was used to disrupt the p53-MDM2 interaction in a concentration-dependent manner, and the results were analyzed and graphed. i3x. 1. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. i3x. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SpectraMax Mini: 17. We would like to show you a description here but the site won’t allow us. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. 3. Quantiferon-TB Gold in Tube Kit. Whether you are looking for an intuitive, easy-to-use reader for your busy,. TaqMan SNP. #R11490)The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as a 10-fold serial dilution series . 2 only) SpectraMax M-series Microplate readers. The isolated EVs were mixed with 1. How to view results from a plate read on the SpectraMax iD5 Hybrid Microplate Reader使用配备SpectraMax注射器卡盒的SpectraMax iD3或 iD5读板机获得激动剂浓度-反应曲线，每次获得一种激动剂 浓度。从低浓度到高浓度对每条浓度-反应曲线进行分析测 定，以减少残留。用化合物灌注注射器，然后将装有染料的Download PDF. The SpectraMax iD3 reader detects fluorescence using monochromators only. Time-resolved phosphorescence. SpectraMax L: 36. 1. The solution was reacted forThe SpectraMax iD5 reader offers an ultra-cooled PMT for background noise reduction, enabling increased sensitivity and dynamic range. Table 2. The Molecular Devices SpectraMax® iD5 Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. 020 fmol 0. Find regional support. Unplug the power cable from the back of the instrument. Statistics; Terms & Conditions; FAQs; Sitemap; News; Contacts; BECOME A PARTNER; EN. The absorbance was measured using a Spectra Max iD5 device (Molecular Devices Inc. Agonist EC 50 values obtained from Calcium 6-QF dye-loaded THP-1 cells. Unparalleled performance on a personalized platform. Italic and boldface type are used for emphasis. Heading. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. SpectraMax iD5: 81. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The Ex/Em of Texas-Cir-amplifier-BHQ2 was 570/615 nm. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. SpectraMax Mini: 17. Instrument setup. 96 mL Au@PLL-AEDP-FITC. 1. Request a Quote! Five-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescenc. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. SpectraMax M5e: 118. BMG LABTECH - CLARIOstar Plus . 8 x 10-15. Example: [Drawer]. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax Paradigm Fluorescence Intensity (FI) Detection Cartridge uses an ultra high power LED for the excitation of various fluorescent labels (see Measurement Specifications on page 19), enabling fluorescence intensity detection methods. Designed to provide optimal performance when used with PerkinElmer’s BRET2 reagents. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12Subsequent samples were measured using a Spectra Max ID5 system (Molecular Devices, Silicon Valley, CA, USA). book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. This process should take about 3 - 5 minutes. Using the SpectraMax iD3 and iD5 readers with the SpectraMax Injector Cartridge with SmartInject Technology, and FLIPR Calcium 6 and 6-QF Assay Kits, we have demonstrated that it is possible to. We used a Cytotoxicity LDH Assay Kit-WST (Dojindo Molecular Technologies, Inc. 8. It may be named "Pothos". The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 2 x 10-14. The pH-Xtra Glycolysis Assay can be measured using filter- or monochromator-based optics on the SpectraMax iD5. 4. Find regional support. techsupport. Plate chamber design of the SpectraMax category 1 microplate readers. ; The top 3 importers of Spectramax are India with 365. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. Eppendorf Eppendorf Assay/Reader Microplates. SpectraMax® iD3 SpectraMax® iD5 SpectraMax® i3x ABSFL LUMI CELL IMAGING WESTERN BLOT INJECTOR TRF FP ALPHA-SCREEN FRET ABS. 100-μL samples ranging from 1 nM to 10 μM were added to triplicate wells of a solid white 96-well microplate. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 50. The SpectraMax iD5 reader offers an ultra-cooled PMT for background noise reduction, enabling increased sensitivity and dynamic range. Heading. Tristar 5 Multimode Microplate Reader. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. No accessories are needed and SoftMax® Pro Software. 6 x 10-15. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Microplate Reader Shelf. SpectraMax® iD3 SpectraMax® iD5 SpectraMax® i3x ABSFL LUMI CELL IMAGING WESTERN BLOT INJECTOR TRF FP ALPHA-SCREEN FRET ABS. The multimode microplate reader SpectraMax iD5 comes with all the relevant optical-sensing measuring technologies built in and can be expanded to include. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 303 unable to cal dark current. Table 2. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD5 reader is. Instrument pricing. spr SpectraMax M2 M2e M3 M4 M5 M5e. ALP 004-Altitude Lab Exposure Control Plan. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. This assay uses the Biuret reaction, in which Cu 2+ is reduced to Cu 1+ by protein in an alkaline medium. Within these modes end-point, well-scan. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalStreamline BCA-based protein quantitation on the SpectraMax iD5 reader. 3. Spectranet customer portal SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 1. Titrated doses of NECA were added to TCR-δ-/-responder T cells cultured in 1 ng/ml of recombinant IL-2. After the incubation. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. Here we show how the DuoLuc reporter assay and SpectraMax iD5 reader are used to detect nuclear factor-κB (NF-κB) activation in a mammalian cell model. 3 and higher include optimized reader settings, as well as data analysis, for faster results. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. The add-incubate-read format enables results in less than one hour and is amenable to. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. 312 gain calibration failed. Application Note: Streamline BCA-Based Protein Quantitation on the SpectraMax iD5 Reader. Terms and Conditions Privacy Policy Online Terms of Use. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. At Spinco, we are committed to excellence in every function of our business – leveraging people, knowledge, technology and innovation to provide products of the highest quality and world class services. 1. Introduction. SpectraMax iD5 reader, which performed FP measurement on the Valita Titer assay plate. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. Lab automation for high-throughput plate-based assays. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Examples: “Press carefully to engage,” “ Do not press down. book Page 1 Wednesday, July 7, 2010 2:09 PM The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. 3) At the bottom of the strip, click on "Show. RIDASCREEN Gliadin competitive ELISA. With optimized reagents, option to operate the readers. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax ® Pro Software. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 3. 3. The absorbance was measured at a wavelength of 405 nm (SpectraMax iD5 multi-mode microplate reader, Molecular Devices, USA). +44 118 944 8000. HEK293 cells were plated into several 96-well cell culture-treated microplates at 10,000 cells per well, and the cells were allowed to recover in a 37°C incubator for 24 hours. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Restart the system. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. In addition, the iD5 reader measures TRF and FP and. Perform the following steps to install the drivers: Start > Control Panel > System. Both readers feature an 8-channel read head for fast reads and. 305 reference level saturation. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Explore how the SpectraMax i3 Multi-Mode Platform provides an unlimited breadth of application possibilities, and how it can evolve with your future needs. 003 fmol 0. We show how the DuoLuc reporter assay and SpectraMax iD5 reader are used to detect nuclear factor-κB (NF-κB) activation in a mammalian cell model. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. com +1 407 99 44 619. 1. Events Calendar. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Fluorescence detection was performed on a SpectraMax iD5 multi-mode microplate reader (Molecular Devices). SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Unlock your i3/i3x instrument after shipment. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. If no sound is heard during initialization, contact technical support. All data are in μM and are n ≥ 3 independent experiments. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 6 μM C12-FAM in a reaction buffer composed of 1 mM HEPES (pH 8) and 1. #iD5-STD) Methods ATP standard curve. Agonist EC 50 values obtained from Calcium 6-QF dye-loaded THP-1 cells. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 7°C and the bottom to 36. The following accessories are required: Set of 2. The. 8. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Prepare your iD series instrument for shipment. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The BCA assay is a two-step colorimetric assay used to quantitate the total protein in a sample. About the reader setup recommendations HTRF assays are available in three different donor/acceptor fluorophore combinations, each requiring specific reader settings for optimal results: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Member since: 2020. BMG LABTECH - CLARIOstar Plus . SpectraMax L: 36. The SpectraMaxiD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes -Easily set up protocols, run experiments,. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. 2 x 10-14.